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 iv) Nitrites and nitrates: it causes blue color of skin because nitrites binds to hemoglobin, so it chemically altered hemoglobin which results in impair oxygen delivery to tissue. This disease is known as blue baby syndrome. Higher amount of nitrates and nitrites consuming can cause cancer and brain tumors.

 v) Annatto: annatto is safe if it is consumes in small amount. Otherwise, it can cause allergies to people who are sensitive to it.

1.10.4 Safety of Food Additives and Preservatives

The limitation should be followed with due importance to factor. The SCF has introduced some guidelines of some test that must be carried out on food additives in order to their safety. It requires numbers of animals and other testes to access every conceivable risk to the consumer:

 • Study for understanding how the body absorbs, metabolizes, and eliminates the substance.

 • Study for potential of gene and chromosome damages.

 • Life time studies and potential of fertility and birth defects.

 • Study of causing cancer.

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