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A summary of all cultures made in cases of uncomplicated influenza is presented in Table III.

Table IIIPresence of B. Influenzæ in Cases of InfluenzaMETHODNUMBER OF CASES CULTUREDB. INFLUENZÆ FOUNDNUMBERPER CENTNose culture28621.4Throat culture16610965.7Sputum culture281760.7Sputum (mouse passage)766180.3Combined nose, throat and sputum cultures and sputum inoculation2828100Combined throat cultures and sputum inoculation4848100

Of any single method used the intraperitoneal inoculation of a white mouse with a specimen of the patient’s sputum proved the most efficient in demonstrating the presence of B. influenzæ. No single method served to demonstrate B. influenzæ in all cases, but by simultaneous cultures from the nose, throat, and deeper air passages no difficulty was met in showing that B. influenzæ was invariably present, usually in abundance somewhere in the respiratory tract during the acute stage of the disease. This result is not out of harmony with the rapidly progressive character of the attack upon the mucous membranes of the respiratory tract in influenza.