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Study of secondary sexual characters is bound up with consideration of the effect of internal secretions of the genital glands on general metabolism. The relation of this influence or its absence (as in the case of artificially castrated animals) has been traced out in the degree of development of the secondary characters. The internal secretions, however, undoubtedly have an influence on all the cells of the body. This is clearly shown by the changes which occur at puberty in all parts of the body, and not only in the seats of the secondary sexual characters. As a matter of fact, the internal secretions of all the glands must be regarded as affecting all the tissues.

The internal secretions of the genital glands must be regarded as completing the sexuality of the individual. Every cell must be considered as possessing an original sexuality, to which the influence of the internal secretion in sufficient quantity is the final determining condition under the influence of which the cell acquires its final determinate character as male or female.