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Although most vessel sealant device handpieces are designed for single use only, resterilization in veterinary medicine is common. Reuse and resterilization of vessel sealant devices intended for single‐use raises the questions of the effectiveness of sterility and ability to perform safe and effective seals. Kuvaldina et al. [27] evaluated the number of uses, cleaning, and resterilization cycles with low temperature hydrogen peroxide sterilization for two LigaSure handpieces intended for open surgery. Based on the rate of vascular seal failure, they recommend discarding the Small Jaw handpiece after 9 cycles and the Impact handpiece after 16 cycles. Another study found the LigaSure Atlas handpiece formed an effective seal after 15 uses and resterilization cycles with ethylene oxide [26]. Gardeweg et al. [25] compared the reuse and resterilization of LigaSure, Caiman, and marSeal handpieces with a low temperature steaming process with formaldehyde as an active agent and found that LigaSure, Caiman, and marSeal devices all performed reliable seals after multiple uses and sterilizations except for one Caiman handpiece. Five cycles of sterilization were performed in this study. Performance and microbiological safety of the LigaSure 5 mm Maryland handpiece were evaluated after multiple uses and resterilization cycles by Valenzano et al. [31] Eleven of 12 handpieces failed to activate when the jaws were closed, and the activation button was depressed after a mean of 7.7 cycles. In this study, each handpiece was initially washed in tap water and a 2% enzymatic cleaner solution. The jaws of the instrument were soaked in the 2% enzymatic cleaner solution for five minutes and then scrubbed with a sponge and metal brush and air‐dried. The handpiece was pouch packaged and sterilized with a low‐temperature hydrogen peroxide sterilization unit. Using this protocol for cleaning and sterilization, no handpieces had positive cultures [31]. The 3‐mm JustRight^TM vessel sealant device from Bolder Surgical was evaluated for multiple uses and resterilization cycles and was considered safe for use for up to nine reuse and resterilization cycles [32].